Nitric Oxide Metabolomics Core Facility

VASCULAR MEDICINE INSTITUTE | UNIVERSITY OF PITTSBURGH





NO Metabolomics @
Vascular Medicine Institute
E1240 BST
200 Lothrop St
Pittsburgh, PA 15261
412-624-0462

Publications

Chemiluminescense techniques

MacArthur PH, Shiva S, Gladwin MT. (2007). Measurement of circulating nitrite and S-nitrosothiols by reductive chemiluminescense. J Chromatogr B Analyt Technol Biomed Life Sci. 851(1-2):93-105. PMID: 17208057.

Pelletier MM, Kleinbongard P, Ringwood L, Hito R, Hunter CJ, SChechter AN, Gladwin MT, Dejam A. (2006). The measurement of blood plasma nitrite by chemiluminescense: pitfalls and solutions. Free Radic Biol Med. 41(4):541-8. PMID: 16863986.


Nitrite & S-nitrosothiol

MacArthur PH, Shiva S, Gladwin MT. (2007). Measurement of circulating nitrite and S-nitrosothiols by reductive chemiluminescense. J Chromatogr B Analyt Technol Biomed Life Sci. 851(1-2):93-105. PMID: 17208057.

Cosyns SM, Shiva S, Lefebvre RA. (2015). Protective effect of exogenous nitrite in postoperative ileus. Br J Pharmacol. 172(20):4864-74. PMID: 26227770.

Rix PJ, Vick A, Attkins NJ, Barker GE, Bott AW, Alcorn H Jr, Gladwin MT, Shiva S, Bradley S, Hussaini A, Hoye WL, Parsley EL, Masamune H. (2015). Pharmacokinetics, pharmacodynamics, safety, and tolerability of nebulized sodium nitrite (AIR001) following repeat-dose inhalation in healthy subjects. Clin Pharmacokinet. 54(3):261-72. PMID: 25421879. PMCID: PMC4335132.

Neye N, Enigk F, Shiva S, Habazetti H, Plesnila N, Kuppe H, Gladwin MT, Kuebler WM. (2012). Inhalation of NO during myocardial ischemia reduces infarct size and improves cardiac function. Intensive Care Med. 38(8):1381-91. PMID: 22653370.

Zuckerbraun BS, Shiva S, Ifedigbo E, Mathier MA, Mollen KP, Rao J, Bauer PM, Choi JJ, Curtis E, Choi AM, Gladwin MT. (2010). Nitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth musce proliferation via xanthine oxidoreductase-dependent nitric oxide generation. Circulation. 121(1):98-109. PMID: 20026772.

Zhao XJ, Wang L, Shiva S, Tejero J, Myerburg MM, Wang J, Frizzell S, Gladwin MT. (2013). Mechanisms for cellular NO oxidation and nitrite formation in lung epithelial cells. Free Radic Biol Med. 61:428-37. PMID: 23639566. PMCID: PMC3883890.


Nitrate

Zuckerbraun BS, Shiva S, Ifedigbo E, Mathier MA, Mollen KP, Rao J, Bauer PM, Choi JJ, Curtis E, Choi AM, Gladwin MT. (2010). Nitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth musce proliferation via xanthine oxidoreductase-dependent nitric oxide generation. Circulation. 121(1):98-109. PMID: 20026772.

Curtis E, Hsu LL, Noguchi AC, Geary L, Shiva S. (2012). Oxygen regulates tissue nitrite metabolism. Antioxid Redox Signal. 17(7):951-61. PMID: 22098300. PMCID: PMC3411351.

Zhao XJ, Wang L, Shiva S, Tejero J, Myerburg MM, Wang J, Frizzell S, Gladwin MT. (2013). Mechanisms for cellular NO oxidation and nitrite formation in lung epithelial cells. Free Radic Biol Med. 61:428-37. PMID: 23639566. PMCID: PMC3883890.


Iron-nitrosyl concentration

Curtis E, Hsu LL, Noguchi AC, Geary L, Shiva S. (2012). Oxygen regulates tissue nitrite metabolism. Antioxid Redox Signal. 17(7):951-61. PMID: 22098300. PMCID: PMC3411351.


Nitrite/nitrate reductase activity

Curtis E, Hsu LL, Noguchi AC, Geary L, Shiva S. (2012). Oxygen regulates tissue nitrite metabolism. Antioxid Redox Signal. 17(7):951-61. PMID: 22098300. PMCID: PMC3411351.

Tiso M, Tejero J, Basu S, Azarov I Wang X, Simplaceanu V, Frizzell S, Jayaraman T, Geary L, Shapiro C, Ho C, Shiva S, Kim-Shapiro DB, Gladwin MT. (2011). Human neuroglobin functions as a redox-regulated nitrite reductase. J Biol Chem. 286(20):18277-89. PMID: 21296891. PMCID: PMC3093900.

Shiva S, Huang Z, Grubina R, Sun J, Ringwood LA, MacArthur PH, Xu X, Murphey E, Darley-Usmar VM, Gladwin MT. (2007). Deoxymyoglobin is a nitrite reductase that generates nitric oxide and regulates mitochondrial respiration. Circ Res. 100(5):654-61. PMID: 17293481.

Zuckerbraun BS, Shiva S, Ifedigbo E, Mathier MA, Mollen KP, Rao J, Bauer PM, Choi JJ, Curtis E, Choi AM, Gladwin MT. (2010). Nitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth musce proliferation via xanthine oxidoreductase-dependent nitric oxide generation. Circulation. 121(1):98-109. PMID: 20026772.


Measurement of NO synthase expression and activity

Cosyns SM, Shiva S, Lefebvre RA. (2015). Protective effect of exogenous nitrite in postoperative ileus. Br J Pharmacol. 172(20):4864-74. PMID: 26227770.

Cruz JA, Bauer EM, Rodriguez AI, Gangopadhyay A, Zeineh NS, Wang Y, Shiva S, Champion HC, Bauer PM. (2012). Chronic hypoxia induces right failure in caveolin-1-/- mice. Am J Physiol Heart Circ Physiol. 302(12):H2518-27. PMID: 22505641. PMCID: PMC3378264.


S-nitrosation by biotin switch

Wang X, Kettenhofen NJ, Shiva S, Hogg N, Gladwin MT. (2008). Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins. Free Radic Biol Med. 44(7):1362-72. PMID: 18211831. PMCID: PMC2396494.

Kamga Pride C, Mo L, Quesnelle K, Dagda RK, Murillo D, Geary L, Corey C, Portella R, Zharikov S, St Croix C, Maniar S, Chu CT, Khoo NK, Shiva S. (2014). Nitrite activates protein kinase A in normoxia to mediate mitochondrial fusion and tolerance to ischaemia/reperfusion. Cardiovasc Res. 101(1):57-68. PMID: 24081164. PMCID: PMC3868348.