Grant C. Bullock, MD, PhD
Grant Bullock, MD, PhD
Office Phone: 412-624-7523
Dr. Bullock is a member of the Department of Pathology, Division of Hematopathology, a Principal Investigator in the Vascular Medicine Institute, and a faculty member in the Cellular and Molecular Pathology Training Program.
BS - University of California, Davis, 1992
MD - University of Iowa, 2001
PhD - University of Iowa, 2001
American Board of Pathology - Clinical Pathology & Hematology
Hematopathology, Molecular Hematopathology, Hematopoiesis, Iron Metabolism
Diagnostic Hematopathology, flow cytometry, molecular diagnostics, molecular cytogenetics applied to hematologic diseases and special coagulation laboratory
My research lab is interested in disorders of blood cell production and function in human hematologic diseases with a major focus on the influence of iron deficiency/sequestration on erythropoiesis in a variety of anemias. Current projects are investigating the effects of iron restriction on mitochondrial function in hematopoietic progenitor cells during erythropoiesis and megakaryopoiesis using a human cell culture models of iron deficiency anemia and mouse models of anemia. We are investigating how iron deficiency suppresses red blood cell production and increases platelet production. We have found evidence that supports an iron-dependent, mitochondrial signaling pathway that involves aconitase enzymes, reactive oxygen species, PKC, ERK, AKT and the distal portion of the erythropoietin receptor. We are investigating the molecular mechanisms of iron sensing by the mitochondria in early erythroid progenitor cells and how this iron sensor affects cell fate and erythropoietin-dependent erythropoiesis. Additionally, through several collaborations within the Vascular Medicine Institute and the University of Pittsburgh, we are investigating: the role of TSP1 and erythroid CD47 in the regulation of cell shape, stability and life-span during sickle cell disease and red blood cell storage; the role of NRF2 in anemia and stress erythropoiesis; the role of guanyl cyclase in hemoglobin F production; the role of erythroid IL33 in erythropoiesis and the sterile inflammation caused by hemolytic anemia; and the role of HMGB1 in megakaryopoiesis and platelet production. The long-term goals of the laboratory are to understand how intrinsic and extrinsic factors regulate red blood cell and platelet production and survival in patients with anemia and use this knowledge to improve therapy for these patients.
Wei J, Zhao J, Schrott V, Zhang Y, Gladwin M, Bullock G*, Zhao Y*, (* co-last authors). Red blood cells store and release interleukin-33. Journal of Investigative Medicine, Accepted for publication 4/7/2015 JIM-D-14-00254R2.
Stowman AM, Hsia LL, Kanner WA, Mahadevan MS, Bullock GC, Patterson JW. Multiple Cutaneous Lymphoproliferative Disorders Showing a Retained Tumor Clone by TCR Gene Rearrangement Analysis: A Case Series of 4 Patients and Review of the Literature. Int J Dermatol 2014 Accepted for publication August 25, 2014. IJD-2014-0765.R4.
Cathro HP, Bullock GC, Bonatti H, Meriden Z, Cook S, Aguilera N. Post-transplant lymphoproliferative disorders are not associated with IgG4 sclerosing disease. Transpl Infect Dis 2014 Oct9. Doi: 10.1111/did. 12300. PMID: 25298125.
Richardson CL, Delehanty LL, Bullock GC, Rival CM, Tung KS, Kimpel DL, et al. Isocitrate ameliorates anemia by suppressing the erythroid iron restriction response. J Clin Invest. 2013 Aug 1;123(8):3614-3623. PMID: 23863711.
Delehanty LL, Bullock GC, Goldfarb AN: Protein Kinase D-HDAC5 Signaling Regulates Erythropoiesis and Contributes to Erythropoietin Cross-talk with GATA1. Blood 120(20):4219-28. PMID: 22983445.
Talbot AL, Bullock GC, Delehanty LL, Sattler M, Zhao ZJ, Goldfarb AN. Aconitase Regulation of Erythropoiesis Correlates with a Novel Licensing Function in Erythropoietin-induced ERK Signaling. PLoS ONE 6(8):e23850. Epub 2011. PMID:21887333.
Bullock GC, Delehanty LL, Talbot AL, Gonias SL, Tong WH, Rouault TA, Dewar B, Macdonald JM, Chruma JJ, Goldfarb AN. Iron Control of Erythroid Development by a Novel Aconitase-associated Regulatory Pathway. Blood. 2010;116:97-108. PMCID: PMC2904585.
Meriden Z, Bullock GC, Bagg A, Bonatti H, Cousar JB, Lopes B, Robbins NK, Cathro HP. Post-transplantation Lymphoproliferative Disease Involving the Pituitary Gland. Human Pathology. 2010;41:1641-1645.
Elagib KE, Mihaylov IS, Delehanty LL, Bullock GC, Ouma KD, Caronia JF, Gonias SL, Goldfarb AN. Cross-talk of GATA-1 and P-TEFb in Megakaryocyte Differentiation. Blood. 2008;112:4884-4894. PMCID: PMC2597596.
Mikesh LM, Crowe SE, Bullock GC, Taylor NE, Bruns DE. Celiac Disease Refractory to a Gluten-free Diet? Clinical Chemistry. 2008;54:441-444.
Haverstick DM, Bullock GC, Bruns DE. Genotyping of Hepatitis C Virus by Melting Curve Analysis: Analytical Characteristics and Performance. Clinical Chemistry. 2004;50(12):2405-2407.
Bullock GC, Bruns DE, Haverstick DM. Hepatitis C Genotype Determination by Melting Curve Analysis with a Single Set of Fluorescence Resonance Energy Transfer Probes. Clinical Chemistry. 2002;48(12):2147-2154.
Bullock GC, Thrower AR, Stinski MF. Cellular Proteins Bind to Sequence Motifs in the R1 Element Between the HCMV Immune Evasion Genes. Experimental & Molecular Pathology. 2002;72(3):196-206.
Bullock GC, Lashmit PE, Stinski MF. Effect of the R1 Element on Expression of the US3 and US6 Immune Evasion Genes of Human Cytomegalovirus. Virology. 2001;288(1):164-174.
Lashmit PE, Stinski MF, Murphy EA, Bullock GC. A cis Repression Sequence Adjacent to the Transcription Start Site of the Human Cytomegalovirus US3 Gene is Required to Down Regulate Gene Expression at Early and Late Times After Infection. Journal of Virology. 1998;72(12):9575-9584.
Thrower AR, Bullock GC, Bissell JE, Stinski MF. Regulation of a Human Cytomegalovirus Immediate-early Gene (US3) by a Silencer-Enhancer Combination. Journal of Virology. 1996;70(1):91-100.
Silverman LM, Bullock GC. Essential Concepts in Molecular Pathology. Ed. W.B. Coleman and G.J. Tsongalis Chapter 29 Molecular Diagnosis of Human Disease. Elsivier, New York, 2010. ISBN: 9780123744180.
Silverman LM, Bullock GC. Molecular Pathology: The Molecular Basis of Human Disease. Ed. W.B. Coleman and G.J. Tsongalis Chapter 28: Molecular Diagnosis of Human Disease. Elsevier, New York, 2009. ISBN: 9780123744197.