UPMC HEART AND VASCULAR INSTITUTE | UNIVERSITY OF PITTSBURGH | FIND PEOPLE
VMI
 


Vascular Medicine Institute
University of Pittsburgh
BST E1240
200 Lothrop Street
Pittsburgh, PA 15261
Phone: 412-383-5853
Fax: 412-648-5980

Hypoxia Core

Print Page
 
1. The animal hypoxic core is located in the SBST 10th floor animal facility.

To minimize animal transfer between facilities, please be sure that SBST 10 location is in your IACUC protocol - this will allow you to order animals directly to SBST 10.

Please contact Dr. Elena Goncharova (eag59@pitt.edu) if you have any questions. 



2. To reserve animal hypoxic chambers for your experiment:

Log into Google Calendar using the egdglab account in order to view the calendars and make changes using password: animalcore

Once logged in, you may view the individual chamber calendars by clicking through the items on the left sidebar. 

The title of each reservation must follow this format: “PI-Last-Name/User’s Name” (without quotes – for example, Gladwin/Courtney). 



3. If you need hemodynamic measurements to be done by the hemodynamic core after hypoxia exposure, please check the core’s surgeons’ availability and sign in ahead of time.

For this, log into Google Calendar using the info below:

Jeff Baust:
Username: vmiphenotypingcore
Password: research1

Jian Hu, MD:
Username: vmicorejian
Password: vmianimalcore

If you are new investigator for the hemodynamic core, please contact Dr. Ana Mora first (anamora@pitt.edu) to coordinate and to confirm that you have an approved IACUC protocol to perform the measurements.



4. Before transferring to the hypoxic chambers, animals should be re-located to the filter-top cages.

Please contact Nicole Scales, SBST Site Supervisor (nls47@pitt.edu), 6-10 business days before starting experiment and provide her with info on how many animals are about to be re-located to ensure that the proper number of such cages will be ready. Minimum of 48 hours is required for the transfer.



5. Please contact us two days before starting your experiment – we will pre-calibrate the system for you.

Please contact Elena Goncharova (eag59@pitt.edu) and/or Dmitry Goncharov (dmg87@pitt.edu).



6. To prevent over-booking, if we see no animals in pre-booked hypoxic chamber not only at the start day, but even 2 days past scheduled start date and the reservation wasn’t modified by user, this reservation will be canceled by core personnel.


7. Unfortunately, DLAR staff can’t provide animal care for animals under hypoxic exposure.

Researchers should care for those animals themselves – this includes monitoring and replenishing of food and water, change of bedding, monitoring animal health and well-being, and filling in the animal husbandry log regularly.



8. Please be sure that your emergency contact info is available to DLAR staff.

Please make sure that your contact emergency information is available to DLAR staff and easy to locate (best way is to print it and stick printout to the cubicle room door – cubicle/chamber numbers should be included).



9. Nitrogen tank rotation:

The low level of oxygen in hypoxic chambers is maintained by automated addition of nitrogen gas.

Based on our previous experience, 3 nitrogen tanks/week are required to keep constant 10% oxygen levels in one chamber (5 tanks for rats).  

PI lab members are responsible for transferring nitrogen tanks between BST12 and SBST10, monitoring nitrogen levels in tanks attached to their chambers, and changing tanks attached to their chambers as needed.

We will have two nitrogen tanks per chamber in vivarium to start with.

We will keep nitrogen tanks supply at BST12, but space is limited and timely exchange of empty tanks is needed. Please return empty tanks back to BST12 by 3 pm on the day you changed it. Please do not wait till both tanks in vivarium will be empty to ensure never-ending nitrogen supply and prompt tank rotation.

Please contact Dr. Charles McTiernan (mctiernanc@upmc.edu) with any nitrogen questions.